|
CABRI:ICLC |
|
Accession_number |
ICLC HYL98004
|
|
Cell_line_name |
B9
|
|
Brief_description |
Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma; Derived from: B13.29 (first described as clone 9.9)
|
|
Description |
Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: hybridoma; Derived from: B13.29 (first described as clone 9.9)
|
|
Depositor |
Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
|
|
Bibliographic_reference |
Eur J Immunol 1987;17:1411-1416 [PMID: 3500054
] |
|
Morphology |
lymphoblast, grown in suspension
|
|
Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50uM 2-mercaptoethanol + 50pg/ml human IL-6 ; maintain cultures between 3-9 x 10^5 cells/ml; split 1:3-1:4; 37C, 5% CO2
|
|
Viruses |
Search for viruses was not performed
|
|
Properties |
fusion partner: SP2/0-Ag14; non producer hybridoma; IL-6 dependent
|
|
Release_conditions |
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
|
|
Hazard |
-
|
|
Species_validation |
Validated by isoenzymes: confirmed as mouse with AST, NP
|
|
Freezing_medium |
90% FBS + 10% DMSO
|
|
Sterility |
mycoplasma negative, HOECHST and PCR
|
|
Comments |
the cells grow very slowly, particularly after thawing
|